Delivery of Molecules into Human Corneal Endothelial Cells by Carbon Nanoparticles Activated by Femtosecond Laser

摘要: Corneal endothelial cells (CECs) form a monolayer at the innermost face of cornea and are engine corneal transparency. Nevertheless, they vulnerable population incapable regeneration in humans, their diseases responsible for one third grafts performed worldwide. Donor corneas stored eye banks security quality controls, then delivered to surgeons. This period could allow specific interventions modify characteristics CECs order increase proliferative capacity, resistance apoptosis, or release immunosuppressive molecules. Delivery molecules specifically into during storage would therefore open up new therapeutic perspectives. For clinical applications, physical methods have more favorable individual general benefit/risk ratio than most biological vectors, but often less efficient. The delivery by carbon nanoparticles activated femtosecond laser pulses is promising recent technique developed on non-adherent cells. partly consummated reaction releasing CO H2 gas bubbles shockwave origin cell transient permeation. Our aim was develop an experimental setting deliver small molecule (calcein) adherent CECs. We confirmed that increased fluence time exposure uptake efficiency while keeping mortality below 5%. optimized area covered beam using motorized stage allowing homogeneous scanning culture surface spiral path. Calcein reached median 54.5% (range 50.3–57.3) with low (0.5%, range (0.55–1.0)). After sorting flow cytometry, having uptaken calcein remained viable presented normal morphological characteristics. prove useful future tissue therapy.