Ostertagia ostertagi Mediates Early Host Immune Responses via Macrophage and Toll-Like Receptor Pathways.

摘要: ABSTRACT Ostertagia ostertagi is an abomasal parasite with significant economic impact on the cattle industry. Early host immune responses are poorly understood. Here, we examined time course expression of Toll-like receptors (TLRs) in peripheral blood mononuclear cells (PBMC) during infection where PBMC macrophages (Mϕ) generated both pro- and anti-inflammatory when incubated excretory/secretory products (ESP) from fourth-stage larvae (OoESP-L4) or adult worms (OoESP-Ad). First, changes cell morphology clearly showed that OoESP-L4 OoESP-Ad activated PBMC-Mϕ vitro, resulting suppressed CD40 increased CD80 expression. Expression mRNAs for TLR1, -4, -5, -7 peaked 7 days postinfection (dpi) (early L4), decreased by 19 dpi (postemergent L4 adults) then at 27 dpi (late adults). The proinflammatory cytokine tumor necrosis factor alpha (TNF-α) (transcript protein) presence OoESP-Ad, interleukin 10 (IL-10) (protein) OoESP-Ad; however, IL-10 mRNA was upregulated, IL-6 downregulated OoESP-L4. When were treated ligands TLR4 TLR5 combination transcripts TNF-α, IL-1, IL-6, significantly relative to treatment only. However, effects TLR2 ligand additive, but only lower concentration. We propose O. utilize competing strategies via TLRs Mϕ confuse system, which allows worm evade innate responses.