Association of the Ras-antagonistic Rap1/Krev-1 proteins with the Golgi complex

摘要: Abstract Ras oncogenes encode 21-kDa GTP-binding proteins that are capable of transforming immortalized cells in culture. Ras bound to the inner face plasma membrane by their C-terminal extremity and thought transmit mitogenic signals via an "effector" domain spanning amino acids 32-42. Two ras-related human genes rap1A rap1B 95% homologous share with p21 same effector a similar Cys-Ali-Ali-Xaa sequence (where Ali is aliphatic acid; also known as CAAX sequence). The product gene identical Krev-1 cDNA, whose overexpression reverting phenotype Ki-ras-transformed NIH 3T3 cells. Antibodies do not cross-react other Ras-related were obtained immunizing rabbits peptide encompassing residues 121-137 Rap1 proteins. These antibodies used investigate subcellular localization indirect immunofluorescence fractionation techniques. found be tightly cellular membranes. They did colocalize on discovered associated Golgi complex.