High-refractive index of acrylate embedding resin clarifies mouse brain tissue.
作者： Hongfu Zhou , Yumiao Xiong , Yu Wang , Xiaojun Wang , Pei Li
DOI: 10.1117/1.JBO.22.11.110503
关键词: Microscopy 、 Polymerization 、 Biophysics 、 Reagent 、 Monomer 、 Fluorescence microscope 、 Chemistry 、 High-refractive-index polymer 、 Fluorescence 、 Acrylate
摘要: Biological tissue transparency combined with light-sheet fluorescence microscopy is a useful method for studying the neural structure of biological tissues. The development also promotes progress in clearing methods. current clarifying methods mostly use liquid reagent to denature protein or remove lipids first, eliminate reduce scattering index refractive tissue. However, denaturing and removing require complex procedures an extended time period. Therefore, here we have developed acrylate resin high index, which causes directly after polymerization. This can improve endogenous retention by adjusting pH value monomer.
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sci-hub.st 参考文章(23)
Douglas S. Richardson, Jeff W. Lichtman, Clarifying Tissue Clearing. Cell. ,vol. 162, pp. 246- 257 ,(2015) , 10.1016/J.CELL.2015.06.067
Sung-Yon Kim, Jae Hun Cho, Evan Murray, Naveed Bakh, Heejin Choi, Kimberly Ohn, Luzdary Ruelas, Austin Hubbert, Meg McCue, Sara L. Vassallo, Philipp J. Keller, Kwanghun Chung, Stochastic electrotransport selectively enhances the transport of highly electromobile molecules Proceedings of the National Academy of Sciences. ,vol. 112, pp. E6274- E6283 ,(2015) , 10.1073/PNAS.1510133112
Bing Hou, Dan Zhang, Shan Zhao, Mengping Wei, Zaifu Yang, Shaoxia Wang, Jiarui Wang, Xin Zhang, Bing Liu, Lingzhong Fan, Yang Li, Zilong Qiu, Chen Zhang, Tianzi Jiang, Scalable and DiI-compatible optical clearance of the mammalian brain Frontiers in Neuroanatomy. ,vol. 9, pp. 19- 19 ,(2015) , 10.3389/FNANA.2015.00019
Ali Ertürk, Klaus Becker, Nina Jährling, Christoph P Mauch, Caroline D Hojer, Jackson G Egen, Farida Hellal, Frank Bradke, Morgan Sheng, Hans-Ulrich Dodt, Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nature Protocols. ,vol. 7, pp. 1983- 1995 ,(2012) , 10.1038/NPROT.2012.119
Kazuki Tainaka, Shimpei I. Kubota, Takeru Q. Suyama, Etsuo A. Susaki, Dimitri Perrin, Maki Ukai-Tadenuma, Hideki Ukai, Hiroki R. Ueda, Whole-body imaging with single-cell resolution by tissue decolorization. Cell. ,vol. 159, pp. 911- 924 ,(2014) , 10.1016/J.CELL.2014.10.034
Yuka Aoyagi, Ryosuke Kawakami, Hisayuki Osanai, Terumasa Hibi, Tomomi Nemoto, A rapid optical clearing protocol using 2,2'-thiodiethanol for microscopic observation of fixed mouse brain. PLOS ONE. ,vol. 10, ,(2015) , 10.1371/JOURNAL.PONE.0116280
T. Kuwajima, A. A. Sitko, P. Bhansali, C. Jurgens, W. Guido, C. Mason, ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue Development. ,vol. 140, pp. 1364- 1368 ,(2013) , 10.1242/DEV.091844
Hiroshi Hama, Hiroshi Kurokawa, Hiroyuki Kawano, Ryoko Ando, Tomomi Shimogori, Hisayori Noda, Kiyoko Fukami, Asako Sakaue-Sawano, Atsushi Miyawaki, Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nature Neuroscience. ,vol. 14, pp. 1481- 1488 ,(2011) , 10.1038/NN.2928
Bi-Chang Chen, Wesley R. Legant, Kai Wang, Lin Shao, Daniel E. Milkie, Michael W. Davidson, Chris Janetopoulos, Xufeng S. Wu, John A. Hammer, Zhe Liu, Brian P. English, Yuko Mimori-Kiyosue, Daniel P. Romero, Alex T. Ritter, Jennifer Lippincott-Schwartz, Lillian Fritz-Laylin, R. Dyche Mullins, Diana M. Mitchell, Joshua N. Bembenek, Anne-Cecile Reymann, Ralph Böhme, Stephan W. Grill, Jennifer T. Wang, Geraldine Seydoux, U. Serdar Tulu, Daniel P. Kiehart, Eric Betzig, Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution Science. ,vol. 346, pp. 1257998- 1257998 ,(2014) , 10.1126/SCIENCE.1257998
Zhongqin Yang, Bihe Hu, Yuhui Zhang, Qingming Luo, Hui Gong, Development of a Plastic Embedding Method for Large-Volume and Fluorescent-Protein-Expressing Tissues PLOS ONE. ,vol. 8, ,(2013) , 10.1371/JOURNAL.PONE.0060877