Clock‐dependent and independent transcriptional control of the two isoforms from the mouse Rorγgene

摘要: Accumulating evidence indicate that molecular mechanisms generating circadian rhythms display some degree of tissue-specificity. More specifically, distinct patterns expression for nuclear receptors the ROR family transcriptional control clock gene Bmal1 differs among tissues. This study aims to investigate Rorγisoforms (Rorγ and Rorγt) characterize underlying their tissue-specific expression. The Rorγ isoforms was assessed in mouse liver, muscle, thymus testis throughout 24 h using quantitative RT-PCR. Although rhythmic liver thymus, it constitutively expressed muscle testis. In contrast, Rorγt constitutive all four Furthermore, impaired Clock mutant mice whereas mutation had no effect on line with these findings, luciferase assays revealed transcription promoter is clock-controlled essentially clock-independent. Our results provide insights into lead differential are suggestive a framework might account regulation.