Four Escherichia coli O157:H7 Phages: A New Bacteriophage Genus and Taxonomic Classification of T1-Like Phages

摘要: The T1-like bacteriophages vB_EcoS_AHP24, AHS24, AHP42 and AKS96 of the family Siphoviridae were shown to lyse common phage types Shiga toxin-producing Escherichia coli O157:H7 (STEC O157:H7), but not non-O157 E. coli. All contained circularly permuted genomes 45.7–46.8 kb (43.8–44 mol% G+C) encoding 74–81 open reading frames 1 arginyl-tRNA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that structural proteins identical among four phages. Further proteomic analysis identified seven responsible for tail fiber, tape measure protein, major capsid, portal protein as well minor proteins. Bioinformatic analyses on AHP24, did encode bacterial virulence factors, integration-related or antibiotic resistance determinants. phages highly lytic STEC with considerable potential biocontrol agents. Comparative genomic, phylogenetic suggested along 17 from database National Center Biotechnology Information (NCBI) can be assigned into a proposed subfamily “Tunavirinae” further classification five genera, namely “Tlslikevirus” (TLS, FSL SP-126), “Kp36likevirus” (KP36, F20), Tunalikevirus (T1, ADB-2 Shf1), “Rtplikevirus” (RTP, vB_EcoS_ACG-M12) “Jk06likevirus” (JK06, vB_EcoS_Rogue1, AHP42, AKS96, phiJLA23, phiKP26, phiEB49). fact viruses related JK06 have been isolated independently in Israel (JK06) (GenBank Assession #, NC_007291), Canada (vB_EcoS_Rogue1, AKS96) Mexico (phiKP26, phiJLA23) (between 2005 2011) indicates these similar are widely distributed, horizontal gene transfer does always prevent characterization bacteriophage evolution. With this new scheme, any discovered same type more properly identified. Genomic- proteomic- based taxonomic would facilitate better understanding diversity genetic traits involved