High resolution mapping using fluorescence in situ hybridization to extended DNA fibers prepared from agarose-embedded cells.
作者： R Karhu , A Palotie , E Hellsten , O P Kallioniemi , L Peltonen
DOI:
关键词: Biophysics 、 Fiber FISH 、 Fluorescence in situ hybridization 、 Molecular biology 、 Agarose 、 Biology 、 Microscope slide 、 Restriction map 、 Fluorescence microscope 、 Positional cloning 、 Molecular probe
摘要: Fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) are essential techniques physical mapping positional cloning. We present a technique that utilizes agarose-embedded high molecular weight DNA prepared for PFGE as target FISH. The agarose blocks melted, the is extended on poly-L-lysine-coated microscope slide. resulting fibers appear slide long straight strands suitable resolution FISH demonstrated here with cosmid plasmid hybridizations.
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