Fluorescence Recovery after Two-Photon Bleaching for the Study of Dye Diffusion in Polymer Systems
作者： Edward Van Keuren , Wolfgang Schrof
DOI: 10.1021/MA034022S
关键词: Chemistry 、 Microscope 、 Calibration curve 、 Photobleaching 、 Fluorescence spectrometry 、 Fluorophore 、 Diffusion 、 Rhodamine 、 Polymer 、 Analytical chemistry
摘要: Fluorescence recovery after two-photon photobleaching (TP-FRAP) was used to characterize the diffusion in polymer thin films. Using a two-photon/confocal microscope, region bleached inside fluorophore-doped poly(vinyl alcohol) film. An ellipsoidal form of this spot observed, and simple derived for fluorescence at center, enabling local fluorophore coefficient be determined. A series films with varying amounts added plasticizer were measured determine relation between content coefficient. In second set inhomogeneous distribution plasticizer, three-dimensional maps using TP-FRAP followed by confocal imaging. From these calibration curves, concentration
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