A complex from cultured Chinese hamster ovary cells containing nine aminoacyl-tRNA synthetases. Thermolabile leucyl-tRNA synthetase from the tsH1 mutant cell line is an integral component of this complex.
作者： Marc MIRANDE , Daniel CORRE , Jean-Pierre WALLER
DOI: 10.1111/J.1432-1033.1985.TB08748.X
关键词: Leucyl-tRNA synthetase 、 Aminoacyl tRNA synthetase 、 Aspartic acid 、 Biochemistry 、 Molecular biology 、 Chinese hamster ovary cell 、 Isoleucine 、 Arginine 、 Thermolabile 、 Glutamine 、 Biology
摘要: The size distribution of the 20 aminoacyl-tRNA synthetses from wild-type Chinese hamster ovary (CHO) cells and mutant cell line tsH1, containing a temperature-sensitive leucyl-tRNA synthetase, was determined by gel filtration. Nine synthetases, specific for arginine, aspartic acid, glutamic glutamine, isoleucine, leucine, lysine, methionine proline, which coeluted as high-Mr entities (Mr∼ 1.2 × 106), were further co-purified to yield multienzyme complex, polypeptide composition identical that previously complex rabbit liver. Immunoprecipitates obtained crude extracts tsH1 cells, using antibodies directed lysyl-tRNA or methionyl-tRNA synthetase components displayed same compositions purified thereby establishing heterotypic nature this complex. Although activity grown at permissive temperature, low compared wild-type, Mr 129000, corresponding enzyme, present in similar amounts occurred exclusively component Finally, we report attempts demonstrate phosphorylation cultured CHO, HeLa C3 unsuccessful.
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