Supramolecular receptor structures in the plasma membrane of lymphocytes revealed by flow cytometric energy transfer, scanning force- and transmission electron-microscopic analyses.

摘要: Receptors in the plasma membrane of blood cells general and that lymphocytes particular are supposed to move around a random walk fashion relatively freely driven by thermal diffusion, as described Singer-Nicolson fluid mosaic model. In this article we summarized data techniques indicated nonrandom codistribution patterns receptor superstructures under conditions, where generation such molecular colocalizations methods themselves were excluded. Application fluorescence energy transfer flow cytometer helped analyze cell populations. After normalizing values for possible differences between labeling ratios targeting monoclonal antibodies using mean distribution curves, two-dimensional maps generated from obtained pair-wise receptors. Major histocompatibility complex (MHC) class I II, intercellular adhesion molecule-1 (ICAM-1), TcR-CD3-CD4, tetraspan molecules (CD81, CD82, CD53), subunits multisubunit IL-2 displayed sometimes with, but very frequently without induction their ligand. Immunogold-bead "sandwich" analyzed atomic force microscopy has shown "islands" existed also "receptor-island-groups". This existence MHC II at an elevated hierarchical level. An analysis is given herein concerning standardized approach. The apparent incompatibility these supramolecular with type "free-protein lipid-mobility paradigm" was resolved recommending additional emphasis on mosaicism besides mobility.