Inhibitory effect of Embelin on human acute T cell lymphoma Jurkat cells through activation of the apoptotic pathway

摘要: It has previously been shown that Embelin inhibits proliferation, promotes apoptosis, and increases sensitivity reduces resistance to chemotherapy drugs, in various types of tumor cells. The present study examined the effects on proliferation human acute T cell lymphoma Jurkat cells were treated with concentrations inhibition growth evaluated. Expression X-linked inhibitor apoptosis protein (XIAP); poly ADP ribose polymerase; caspase-3; caspase-8; caspase-9; proapoptotic protein, Bax; antiapoptotic proteins, Bcl-xl Bcl-2, assessed. results showed significantly inhibited Following treatment 5, 10 or 20 mM for 48 h, viability was 82.31, 58.65 37.62%, respectively, which reduced compared control group 0.1% DMSO (P<0.01). Furthermore, caspase-3 inhibitor, z-DEVD-fmk, caspase-9 Ac-LEHD-CHO, reversed this inhibitory effect. also apoptotic rate elevated. Subsequently, it demonstrated downregulated expression XIAP Bcl2 family members, Bcl-2 Bcl-xl, while concomitantly upregulated Bax. These induced vitro, by activating endogenous caspase-dependent pathway through members.