Bacterial Enhancer Binding Proteins-AAA+ Proteins in Transcription Activation.

摘要: Bacterial enhancer-binding proteins (bEBPs) are specialised transcriptional activators. bEBPs hexameric AAA+ ATPases and use ATPase activities to remodel RNA polymerase (RNAP) complexes that contain the major variant sigma factor, σ54 convert initial closed complex transcription competent open complex. Earlier crystal structures of domains alone have led proposals how nucleotide-bound states sensed propagated substrate interactions. Recently, structure domain a bEBP bound RNAP-σ54-promoter DNA was revealed. Together with complex, an intermediate state where is partially loaded into RNAP cleft promoter mechanistic understanding ATP activate can now be proposed. This review summarises current structural models emerging this special class utilises allow σ54-dependent initiation.