Exogenous hydrogen sulfide attenuates diabetic myocardial injury through cardiac mitochondrial protection
作者： Xin Zhong , Leihong Wang , Yuwen Wang , Shiyun Dong , Xiaoning Leng
DOI: 10.1007/S11010-012-1435-3
关键词: Cytochrome c 、 Nitric oxide 、 Biochemistry 、 Apoptosis 、 Reactive oxygen species 、 Cardiac function curve 、 Internal medicine 、 Sodium hydrosulfide 、 Mitochondrion 、 Streptozotocin 、 Endocrinology 、 Medicine
摘要: In the study, we investigated how exogenous H2S (hydrogen sulfide) influenced streptozotocin (STZ)-induced diabetic myocardial injury through cardiac mitochondrial protection and nitric oxide (NO) synthesis in intact rat hearts primary neonatal cardiomyocytes. Diabetes was induced by STZ (50 mg/kg) daily administration of 100 μM NaHS (sodium hydrosulfide, an donor) diabetes + NaHS treatment group. At end 4, 8, 12 weeks, morphological alterations functions were observed using transmission electron microscopy echocardiography system. The percentage apoptotic cardiomyocytes, membrane potential, production reactive oxygen species (ROS) level NO measured. expressions cystathionine-γ-lyase (CSE), caspase-3 -9, NOX4 cytochrome c analyzed western blotting. results showed function injured, changes rate increased hearts. cardiomyocytes high glucose group, ROS markedly, whereas expression CSE decreased. However, with significantly reversed function, decreased levels administrated Furthermore, down-regulated -9 inhibited release from mitochondria conclusion, is involved attenuation mitochondria.
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