Expression profiling identifies new function of collapsin response mediator protein 4 as a metastasis-suppressor in prostate cancer
作者： X Gao , J Pang , L-Y Li , W-P Liu , J-M Di
DOI: 10.1038/ONC.2010.213
关键词: Downregulation and upregulation 、 Metastasis suppressor 、 Cancer research 、 Cancer 、 Metastasis 、 Prostate cancer 、 Gene expression profiling 、 Immunology 、 Metastasis Suppressor Gene 、 Biology 、 Carcinogenesis
摘要: Metastasis is the chief cause of mortality from cancer, but mechanisms leading to metastasis are poorly understood. We used a proteomics approach screen for metastasis-associated proteins and found that collapsin response mediator protein-4 (CRMP4) expression was inversely associated with lymph node prostate cancer (PCa). Subsequent in vitro vivo studies revealed overexpression CRMP4 not only suppressed invasion ability PCa cells, also strongly inhibited tumor an animal model. Furthermore, methylation CpG island within promoter region gene responsible downregulation expression. Thus, this study, we show new function as metastasis-suppressor PCa. The findings provide mechanistic insights into therapeutic potential most common male cancer.
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sci-hub.se 参考文章(47)
Jin-Yuan Shih, Yuan-Chii G. Lee, Shuenn-Chen Yang, Tse-Ming Hong, Chi-Ying F. Huang, Pan-Chyr Yang, Collapsin response mediator protein-1: A novel invasion-suppressor gene Clinical & Experimental Metastasis. ,vol. 20, pp. 69- 76 ,(2003) , 10.1023/A:1022598604565
C. A. Klein, The Metastasis Cascade Science. ,vol. 321, pp. 1785- 1787 ,(2008) , 10.1126/SCIENCE.1164853
J.CIark Susan, Janet Harrison, Cheryl L. Paul, Marianne Frommer, High sensitivity mapping of methylated cytosines. Nucleic Acids Research. ,vol. 22, pp. 2990- 2997 ,(1994) , 10.1093/NAR/22.15.2990
Denis Martin Schewe, Tobias Biller, Gabriele Maurer, Irfan A. Asangani, Joerg H. Leupold, Ernst R. Lengyel, Stefan Post, Heike Allgayer, Combination analysis of activator protein-1 family members, Sp1 and an activator protein-2α-related factor binding to different regions of the urokinase receptor gene in resected colorectal cancers Clinical Cancer Research. ,vol. 11, pp. 8538- 8548 ,(2005) , 10.1158/1078-0432.CCR-05-0786
Volker Rosslenbroich, Lingsong Dai, Stephan L. Baader, Angelika A. Noegel, Volkmar Gieselmann, Joachim Kappler, Collapsin response mediator protein-4 regulates F-actin bundling. Experimental Cell Research. ,vol. 310, pp. 434- 444 ,(2005) , 10.1016/J.YEXCR.2005.08.005
B W Ozanne, H J Spence, L C McGarry, R F Hennigan, Transcription factors control invasion: AP-1 the first among equals Oncogene. ,vol. 26, pp. 1- 10 ,(2007) , 10.1038/SJ.ONC.1209759
Peri H. Tate, Adrian P. Bird, Effects of DNA methylation on DNA-binding proteins and gene expression. Current Opinion in Genetics & Development. ,vol. 3, pp. 226- 231 ,(1993) , 10.1016/0959-437X(93)90027-M
Hongjun Fu, Wenming Li, Yulin Liu, Yuanzhi Lao, Wei Liu, Cheng Chen, Hua Yu, Nelson T. K. Lee, Donald C. Chang, Peng Li, Yuanping Pang, Karl W. K. Tsim, Mingtao Li, Yifan Han, Mitochondrial proteomic analysis and characterization of the intracellular mechanisms of bis(7)-tacrine in protecting against glutamate-induced excitotoxicity in primary cultured neurons. Journal of Proteome Research. ,vol. 6, pp. 2435- 2446 ,(2007) , 10.1021/PR060615G
C Q Cai, Y Peng, M T Buckley, J Wei, F Chen, L Liebes, W L Gerald, M R Pincus, I Osman, P Lee, Epidermal growth factor receptor activation in prostate cancer by three novel missense mutations Oncogene. ,vol. 27, pp. 3201- 3210 ,(2008) , 10.1038/SJ.ONC.1210983
J. G. Herman, J. R. Graff, S. Myohanen, B. D. Nelkin, S. B. Baylin, Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 9821- 9826 ,(1996) , 10.1073/PNAS.93.18.9821