Action of Phospholipase A at Interfaces
作者： Robert Verger , Maria C.E. Mieras , Gerard H. de Haas
DOI: 10.1016/S0021-9258(19)43833-7
关键词: Ion 、 Enzyme kinetics 、 Penetration (firestop) 、 Thermodynamics 、 Monolayer 、 Chemistry 、 Heterogeneous catalysis 、 Kinetics 、 Stereochemistry 、 Phospholipase A 、 Surface pressure 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Abstract The heterogeneous catalysis of pancreatic phospholipase A (EC 3.1.1.4) is studied by kinetics on monomolecular layers short chain phospholipids. Under certain conditions long induction times are observed experimentally which can be related with a slow reversible penetration the enzyme into monolayer. influence surface pressure, pH, sodium chloride, and Ca2+ ions this step reported. Based proposed two-dimensional Michaelis model, steady state presteady equations derived together computer analysis show good fit experimental results. quantitative comparison between obtained monolayer bulk techniques attempted using common definition "quality" lipid-water interface. Possible implications concept site discussed.
elsevier.com
sci-hub.st 参考文章(28)
R. H. Quarles, R. M. C. Dawson, The hydrolysis of monolayers of phosphatidyl-[Me−14C]choline by phospholipase D Biochemical Journal. ,vol. 113, pp. 697- 705 ,(1969) , 10.1042/BJ1130697
Dennis Chapman, Biological membranes : physical fact and function Academic Press. ,(1968)
G.H. De Haas, P.P.M. Bonsen, W.A. Pieterson, L.L.M. Van Deenen, Studies on phospholipase A and its zymogen from porcine pancreas Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 239, pp. 252- 266 ,(1971) , 10.1016/0005-2760(71)90171-8
Arthur F. Rosenthal, Stella Ching-Hsien Han, A study of phospholipase a inhibition by glycerophosphatide analogs in various systems Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 218, pp. 213- 220 ,(1970) , 10.1016/0005-2760(70)90139-6
E. Cubero Robles, H. de Iongh, Direct acylation of glycerylphosphorylcholine: (Short communication) Recueil des Travaux Chimiques des Pays-Bas. ,vol. 86, pp. 762- 764 ,(2010) , 10.1002/RECL.19670860712
Hans Kuhn, Dietmar Möbius, Systems of Monomolecular Layers—Assembling and Physico‐Chemical Behavior Angewandte Chemie. ,vol. 10, pp. 620- 637 ,(1971) , 10.1002/ANIE.197106201
D. E. Koshland, Application of a Theory of Enzyme Specificity to Protein Synthesis Proceedings of the National Academy of Sciences. ,vol. 44, pp. 98- 104 ,(1958) , 10.1073/PNAS.44.2.98
G. Benzonana, P. Desnuelle, Etude cinetique de l’action de la lipase pancreatique sur des triglycerides en emulsion. Essai d’une enzymologie en milieu heterogene Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation. ,vol. 105, pp. 121- 136 ,(1965) , 10.1016/S0926-6593(65)80181-3
Sidney Fleischer, Herman Klouwen, The role of soluble lipid in mitochondrial enzyme systems Biochemical and Biophysical Research Communications. ,vol. 5, pp. 378- 383 ,(1961) , 10.1016/0006-291X(61)90044-4
Robert Verger, Gerard H. de Haas, Enzyme reactions in a membrane model 1: A new technique to study enzyme reactions in monolayers Chemistry and Physics of Lipids. ,vol. 10, pp. 127- 136 ,(1973) , 10.1016/0009-3084(73)90009-1