MicroRNA-1 and microRNA-206 suppress LXRα-induced lipogenesis in hepatocytes.

摘要: Abstract Liver X receptor α (LXRα) plays a critical role in the transcriptional control of lipid metabolism. LXR activation induces expression lipogenic genes, which promote hepatic steatosis and steatohepatitis. However, regulation is not fully understood. MicroRNAs (miRs) are regarded as important negative regulators gene expression. In this study, we found that miR-1/miR-206 repressed LXRα-induced accumulation droplets hepatocytes. addition, bioinformatic analysis predicted same putative target-site for located within 3′-untranslated region (3′-UTR) LXRα mRNA. The reporter assay revealed directly targeted 3′-UTR Furthermore, at both mRNA protein levels, accompanied with inhibition target such sterol-regulatory element binding 1c, fatty acid synthase, carbohydrate responsive element-binding acetyl-CoA carboxylase 1, effectors implicated lipogenesis. Moreover, ectopic without dramatically attenuated miR-1/miR-206-induced decrease genes droplet accumulation. Taken together, first time demonstrated lipogenesis by targeting mRNA, suggesting miR-1/miR-206-LXRα pathway may be novel treatment lipogenesis-associated diseases.