A Ribosomal RNA Operon and its Flanking Region from the Archaebacterium Methanobacterium thermoautotrophicum, Marburg Strain: Transcription Signals, RNA Structure and Evolutionary Implications

摘要: Summary One of the two ribosomal RNA operons thermophilic methanogen Methanobacterium thermoautotrophicum was sequenced and examined for transcriptional signals limits, gene organization primary secondary structure 16S 5 S RNA. start identified 213 nucleotides upstream from gene, preceded by a long T-rich sequence that could be promoter. Two other sites were 159 91 are either both transcription starts or processing sites. A termination site localized downstream 5S at end run pyrimidines. Both generate universal structures with G-C contents no higher than those non-thermophilic methanogens. Open reading frames border operon lack Shine-Dalgarno sequences exhibit homology known open bordering operons. Phylogenetic analyses confirm methanogens constitute sub-group archaebacteria M. is closely related to formicicum .