Method for assessing biofilms

作者: Dietrich Otto Ruehlmann , Simon Laurence John Stubbs , Rudi Willy Roger Labarbe , Ian David Goodyer

DOI:

关键词: BiofilmSource systemFluorescenceComputer softwareConfocal imagingMaterials scienceRadiationElectromagnetic radiationOpticsAutomated method

摘要: An automated method for measuring the development of a biofilm, containing one or more fluorescent moieties, on plurality surfaces using confocal imaging system including: a) radiation source forming beam electromagnetic including wavelengths; b) an optical directing and focusing said onto planes object; c) detection detecting emitted from object producing image data; d) scanning in with radiation, comprising steps of: i) growing biofilm surfaces; ii) presence moieties within by collecting emissions to produce images; iii) analysing images means data processing under control computer software determine structure biofilm.

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