Proteomic pattern changes associated with obesity‐induced asthenozoospermia

摘要: Obesity, an increasingly frequent societal disease can also be accompanied by declines in spermatozoa quality and male subfecundity. To determine if there are obesity-associated proteomic changes potentially affecting sperm motility, differential analysis was performed on from both asthenozoospermia clinically healthy individuals, using a label-free quantitative LC-MS/MS approach. We resolved 1975 proteins the human proteome, amongst which, 105 were less abundant, whereas 22 other increased asthenozoospermia. Functional category analyses indicated that differentially expressed mainly related to cytoskeletal regulation, vesicle biogenesis, metabolism, protein degradation involved spermiogenesis motility. Furthermore, endoplasmic reticulum 57 (ERp57) actin-binding-related T2 (ACTRT2) expression verified immunofluorescence, Western blot, flow cytometry analyses. It is evident ERp57 localized acrosome region, neck principal piece of spermatozoa, ACTRT2 post-acrosomal region middle piece. Thus, these differences may contribute underlying defects afflicting individuals. Notably, play critical roles reducing