Long-term potentiation during whole-cell recording in rat hippocampal slices.

摘要: Abstract Factors involved in the production of long-term potentiation CA1 region rat hippocampal slices were examined using whole-cell voltage clamp recordings. The pairing postsynaptic membrane depolarization with tetanic stimulation produced a reliable long-lasting enhancement synaptic currents provided that was performed within 15 min after establishing intracellular contact. This time could be extended to 30 by including adenosine triphosphate and guanosine recording pipette. Once established, persisted for 3 h or more. washout generating ability not correlated rundown baseline N -methyl- d -aspartate receptor-mediated component responses, but followed course similar loss calcium spikes. Long-term reliably depolarizing −40 − 20 mV during tetanus, decreased when held at potentials greater than 0mV. At mV, 50 μM 2-amino-5-phosphonovalerate blocked this agent ineffective +40 mV. In contrast, verapamil, channel blocker, failed alter −20 +40mV. These results suggest causes factors important initiation potentiation. However, these are less maintaining Furthermore, depending on potential stimulation, voltage-gated channels contribute