Serum-free mammalian cell culture medium, and uses thereof

摘要: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly suspension, of mammalian cells, epithelial cells and fibroblast methods for cultivating suspension using these media. media comprise basal polyanionic or compound, preferably polysulfonated polysulfated more dextran sulfate. also chemically defined, protein-free eukaryotic comprising an iron chelate zinc, which is capable supporting growth (and high-density cells) culture, increasing level expression recombinant protein cultured and/or virus production cells.