作者: Larry E. Morrison , Garfield P. Royer , Kenneth A. Cruickshank
DOI:
关键词: Polymerase 、 Chemistry 、 Biochemistry 、 Sequence (biology) 、 Covalent bond 、 Product (mathematics) 、 Polynucleotide 、 Ligation 、 Enzyme 、 Stereochemistry 、 RNA-dependent RNA polymerase
摘要: A method for the detection of a target polynucleotide sequence wherein is used as template to direct hybridization first and second probes contiguous portions target. The hybridized are then ligated, preferably by covalent linkage photoreactive functional groups present on each probes, produce ligated reaction product. product multiplied with polymerase such Q-beta replicase an enzyme which detected. presence indicative sequence.