Template-directed ligation and amplification assay

作者: Larry E. Morrison , Garfield P. Royer , Kenneth A. Cruickshank

DOI:

关键词: PolymeraseChemistryBiochemistrySequence (biology)Covalent bondProduct (mathematics)PolynucleotideLigationEnzymeStereochemistryRNA-dependent RNA polymerase

摘要: A method for the detection of a target polynucleotide sequence wherein is used as template to direct hybridization first and second probes contiguous portions target. The hybridized are then ligated, preferably by covalent linkage photoreactive functional groups present on each probes, produce ligated reaction product. product multiplied with polymerase such Q-beta replicase an enzyme which detected. presence indicative sequence.

参考文章(4)
Larry Edward Morrison, Garfield Paul Royer, Kenneth Alexander Cruickshank, Template-directed photoligation ,(1989)
Eleanor Anne Miele, Donald Robert Mills, Fred Russell Kramer, Autocatalytic replication of recombinant RNA ,(1984)
Alexander N. Glazer, Michael W. Hunkapiller, Norman M. Whiteley, Detection of specific sequences in nucleic acids ,(1985)