Improved fusion tag cleavage strategies in the downstream processing of self-assembling virus-like particle vaccines
作者： Yang Wu , Linda H.L. Lua , Anton P.J. Middelberg , Natalie K. Connors
DOI: 10.1016/J.FBP.2013.08.012
关键词: Capsomere 、 Self assembling 、 Bioprocess 、 Computational biology 、 Cleavage (embryo) 、 Murine polyomavirus 、 Biology 、 Downstream processing 、 Thrombin 、 Virology 、 Virus-Like Particle Vaccines
摘要: … No non-specific cleavage sites were identified for … A large amount of non-cleaved GST-EK-VP1 protein is observed … of the monomeric protein should be avoided to ensure the highest-…
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