Administration of alpha-ketoglutarate improves epithelial restitution under stress injury in early-weaning piglets

摘要: // Liuqin He 1, 2, * , Xihong Zhou Niu Huang 3 Huan Li Zhijie Cui 4 Junquan Tian 2 Qian Jiang Shaojuan Liu Jian Wu Tiejun 5 Kang Yao and Yulong Yin 5, 6 1 Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute Agriculture, Chinese Academy Sciences, Scientific Observing Experimental Station Animal Nutrition Feed Science South-Central, Ministry Hunan Provincial Engineering Research Center for Healthy Livestock Poultry Production, Changsha, 410125, China University Beijing, 10039, College Technology, Agricultural University, Hunan, 410128, Xiangtan Xiangtan, 411105, Co-Innovation Production Safety, Human Health, Life Normal 410006, These authors have contributed equally to this work Corresponding to: Yin, email: yinyulong@isa.ac.cn Yao, yaokang@isa.ac.cn Keywords: AKG, inflammatory cytokines, nutrient-sensing transporters, tight-junction proteins, epithelial restitution Received: May 10, 2017     Accepted: August 07, Published: 24, 2017 ABSTRACT Alpha-ketoglutarate (AKG) is an important cellular metabolite that participates energy production amino acid metabolism. However, the protective effects mechanism AKG on mucosal lesions not been well understood. This study was conducted investigate dietary supplementation early-weaning piglets under Escherichia coli lipopolysaccharide (LPS) induction. A total 32 weaned were used a × factorial design; major factors treatment (basal diet or diet) challenge (LPS saline). The results showed improved growth performance intestinal morphology LPS-induced piglets. Compared with basal diet, remarkably decreased concentration mRNA expression cytokines (IL-1β, IL-6, IL-12) Moreover, administration upregulated transporters (GLUT-2, SGLT-1, PEPT-1, I-FABP2) small intestine both saline- LPS-treated piglets, distribution genes andproteins (ZO-1, Occludin, Claudins, E-cadherin). Collectively, our findings indicate has potential alleviate response improve ability stress injury it also provides experimental basis enteral use swine clinical application prevent damage.