SRV2, a gene required for RAS activation of adenylate cyclase in yeast

摘要: We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation cAMP cell. Direct assays indicate diminish rate vivo production following stimulation by allele. Null srv2 result lethality, cannot be suppressed mutational activation cAMP-dependent protein kinase. The sequence indicates it encodes adenylate cyclase-associated protein. These results demonstrate SRV2 is required for RAS-activated cyclase activity, but participates other essential cellular functions as well.