High-performance liquid chromatographic method for pharmacokinetic studies on the new anthracycline 4-demethoxydaunorubicin and its 13-dihydro derivative

摘要: Abstract The anthracyclines are a group of antitumoral antibiotics with significant clinical efficacy. Among the new anthracycline derivatives, 4-demethoxydaunorubicin showed interesting biological properties in terms both spectrum activity and therapeutic index was recently introduced trials. present paper describes analytical method developed to investigate pharmacokinetics this derivative. consists extraction its 13-dihydro metabolite from plasma chloroform—1-heptanol (9:1) re-extraction 0.3 M phosphoric acid, separation by high-performance liquid chromatography quantification sensitive fluorescence detection. Plasma level curves obtained cancer patients treated drug shown.