Molecular characterization of H9N2 avian influenza viruses isolated from vaccinated broiler chickens in northeast Iran
作者： Pejman Bahari , Seyed Ali Pourbakhsh , Hamid Shoushtari , Mohammad Ali Bahmaninejad
DOI: 10.1007/S11250-015-0848-X
关键词: Antigenic drift 、 Sequence analysis 、 H5N1 genetic structure 、 Biology 、 Antigenic shift 、 Virology 、 Influenza A virus subtype H5N1 、 Neuraminidase 、 Influenza A virus 、 Vaccination 、 Microbiology
摘要: Avian influenza is a highly contagious respiratory disease of poultry caused by A viruses, family Orthomyxoviridae. H9N2 avian outbreaks are major problem the industry in Iran. To determine genetic differences between field viruses and vaccine strain, genomes four strains isolated 2011 from vaccinated broiler flocks with history illness were sequenced. Genetic serological comparisons made. Sequence analysis hemagglutinin (HA) neuraminidase (NA) genes indicated that shared nucleotide homologies 91.6-93.9 90.2-91.7% respectively. Phylogenetic analyses HA NA showed all this study fell into same group belonged to virus (A)/quail/Hong Kong/G1/97 sublineage. Several amino acids have changed at antigenic sites viruses. Extra potential glycosylation observed proteins expressed current isolates relative those strain. The deduced acid sequence cleavage site recent KSSR/GLF motif, whereas it RSSR/GLF revealed currently circulating antigenically distinct These results suggest commercial insufficiently genetically similar region. findings confirm important monitor variations when selecting
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