Effects of chlortetracycline on soil microbial communities: Comparisons of enzyme activities to the functional diversity via Biolog EcoPlates™

摘要: Abstract Veterinary antibiotics (VAs) can enter agricultural soil by the application of animal excrements. However, response microorganisms to VAs is not well understood. Therefore, a 45-day microcosm experiment was conducted ascertain impact chlortetracycline (CTC) on microbial communities. In this study, CTC applied samples with no previous antibiotic-containing manure history at three concentrations 1, 10, and 100 mg/kg soil. Soil were taken after 6, 12 45 day(s) incubation determine dissipation using HPLC. Meanwhile, investigate effect microorganisms, activities in cultivable actual community determined Biolog EcoPlates™ enzyme activities, respectively. It demonstrated that dissipated faster lower spiking levels. At end experiment, could be extracted all treatments, except for C100. The enhanced first day then significantly inhibited up days. This inhibition especially true dehydrogenase urease, whereas phosphatase slightly affected. results contamination pose selective pressure despite its rapid decline extractable concentration. community-level physiological profiles (CLPP) assessed displayed different susceptibilities addition CTC: it responded rapidly CTC-amendment improved strongly day, detected thereafter. suggested EcoPlates method more suitable short-term toxicity tests than relatively long-term effects pollutants