Application of dispersive liquid-liquid microextraction coupled with vortex-assisted hydrophobic magnetic nanoparticles based solid-phase extraction for determination of aflatoxin M1 in milk samples by sensitive micelle enhanced spectrofluorimetry.

摘要: Abstract An efficient, simple and fast low-density solvent based dispersive liquid–liquid microextraction (LDS-DLLME) followed by vortex-assisted solid phase extraction (VA-D-SPE) has been developed as a new approach for preconcentration of aflatoxin M1 in milk samples prior to its micelle enhanced spectrofluorimetic determination. In this LDS-DLLME coupled VA-D-SPE method, were first treated with methanol/water (80:20, v/v) after removing the fat layer. This was directly used dispersing DLLME along using 1-heptanol (as respect water) extracting solvent. approach, hydrophobic oleic acid modified Fe3O4 nanoparticles retrieve analyte from step. It is considerably that target rather than directly. The main parameters affecting efficiency procedures signal enhancement investigated optimized. Under optimum conditions, method linear range 0.02 200 µg L−1 correlation coefficient (R2) 0.9989 detection limit 13 ng L−1. intra-day precision 2.9 4.3% inter-day 2.1 3.3% concentration 2 50 µg L−1 respectively. applied AFM1 three commercially available results compared official AOAC method.