Maturation, fertilization, and development of marmoset monkey oocytes in vitro.

摘要: This study was conducted to investigate 1) the capacity of in vitro-matured (IVM) marmoset oocytes be fertilized and support embryonic development vitro 2) oocyte meiotic maturation relation vivo FSH administration, follicle size, oocyte-cumulus cell status. Pairs ovaries were collected on Day 4 follicular phase from adult females receiving either human (3 IU; n = 5) or control (saline; daily for days. Antral follicles excised separated into classes according size: class 1 (660-840 microm), 2 (> 840-1000 3 1000-1400 1400 microm). A total 823 partially naked cumulus-enclosed (CEOs) released cultured vitro. Cumulus cells remaining after 22 h removed, metaphase II (MII) inseminated with epididymal sperm, resulting embryos until developmental arrest. Fluorescence microscopy used assess embryo progression. Oocyte germinal vesicle breakdown (GVB)- MII-competencies increased significantly size (p < 0.01 p 0.0001, respectively), although they independent associations. After 24 32 vitro, 69% 93%, respectively, CEOs MII competence had completed maturation, rate nuclear progressively 0.01) association cumulus 0.01). In priming slightly improved GVB- 0.05, respectively) decreased time required achieve IVM all sizes (78-92%) 27% developing morula- 4% blastocyst-stage embryos. demonstrates first that New World primate are able advanced preimplantation course profoundly influenced by their origin, marginally treatment.