Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes.

作者: F. Zhu , W. Yan , Z.L. Zhao , Y.B. Chai , F. Lu

DOI: 10.2144/00292ST06

关键词: Clone (cell biology)ApoptosisSuppression subtractive hybridizationGeneCloningRetinoic acidDifferentially expressed genesBiologyMolecular biologyPolymerase chain reaction

摘要: An improved PCR-based subtractive hybridization strategy was used to clone apoptosis-related genes induced by all-trans retinoic acid (ATRA) from human promyelocytic leukemia cell line HL-60 cells....

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