Evaluation of different detection modes for the analysis of procyanidins in leaves and flowers ofCrataegus spp. Part I. Diode array and electrochemical detection
作者： Gabriela E. Rohr , Beat Meier , Otto Sticher
DOI: 10.1002/(SICI)1099-1565(200003/04)11:2<106::AID-PCA496>3.0.CO;2-O
关键词: High-performance liquid chromatography 、 Chemistry 、 Linearity 、 Monomer 、 Selectivity 、 Chromatography 、 Degree of polymerization 、 Analytical chemistry 、 Absorbance 、 Matrix (chemical analysis) 、 Quantitative analysis (chemistry)
摘要: Diode array and single electrode electrochemical detection were evaluated for their use in the analysis of (−)-epicatechin oligomeric procyanidins reference solutions extracts leaves flowers Crataegus spp. Linearity regression curves was good both modes. found to be more selective but less sensitive than detection. The absorbance ratio 220/280 nm gives information on degree polymerization solutions. In presence matrix no additional structural can obtained from ratios due high standard deviations. Quantitative evaluation terms monomer equivalents using leads an underestimation contents procyanidin dimers, it is applicable at 280 nm. view better selectivity easier handling, diode recommended complex matrices. Copyright © 2000 John Wiley & Sons, Ltd.
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sci-hub.se 参考文章(28)
Lawrence J. Porter, Structure and Chemical Properties of the Condensed Tannins Springer, Boston, MA. pp. 245- 258 ,(1992) , 10.1007/978-1-4615-3476-1_14
Gabriela Elisabeth Rohr, Analytical investigation on and isolation of procyanidins from Crataegus leaves and flowers ETH Zürich. ,(1999) , 10.3929/ETHZ-A-002045003
G.E. Rohr, B. Meier, O. Sticher, Analysis of procyanidins Studies in natural products chemistry. ,vol. 21, pp. 497- 570 ,(2000) , 10.1016/S1572-5995(00)80013-7
Lesley J. Putman, Larry G. Butler, Separation of high molecular weight sorghum procyanidins by high-performance liquid chromatography Journal of Agricultural and Food Chemistry. ,vol. 37, pp. 943- 946 ,(1989) , 10.1021/JF00088A025
Begoña Bartolomé, Isabel Estrella, T. Hernández, Changes in phenolic compounds in lentils (Lens culinaris) during germination and fermentation Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A, European food research and technology. ,vol. 205, pp. 290- 294 ,(1997) , 10.1007/S002170050167
R. S. Thompson, D. Jacques, E. Haslam, R. J. N. Tanner, Plant proanthocyanidins. Part I. Introduction; the isolation, structure, and distribution in nature of plant procyanidins Journal of the Chemical Society, Perkin Transactions 1. pp. 1387- 1399 ,(1972) , 10.1039/P19720001387
Teresa. Escribano-Bailon, Yolanda. Gutierrez-Fernandez, Julian C. Rivas-Gonzalo, Celestino. Santos-Buelga, Characterization of procyanidins of Vitis vinifera variety Tinta del Pais grape seeds Journal of Agricultural and Food Chemistry. ,vol. 40, pp. 1794- 1799 ,(1992) , 10.1021/JF00022A013
D. Treutter, C. Santos-Buelga, M. Gutmann, H. Kolodziej, Identification of flavan-3-ols and procyanidins by high-performance liquid chromatography and chemical reaction detection Journal of Chromatography A. ,vol. 667, pp. 290- 297 ,(1994) , 10.1016/0021-9673(94)89078-1
Andrew G.H. Lea, Reversed-phase gradient high-performance liquid chromatography of procyanidins and their oxidation products in ciders and wines, optimised by Snyder's procedures Journal of Chromatography A. ,vol. 194, pp. 62- 68 ,(1980) , 10.1016/S0021-9673(00)81050-X
Gabriela E Rohr, Beat Meier, Otto Sticher, Quantitative reversed-phase high-performance liquid chromatography of procyanidins in Crataegus leaves and flowers Journal of Chromatography A. ,vol. 835, pp. 59- 65 ,(1999) , 10.1016/S0021-9673(99)00043-6