Tailor-Making a Protein A-Derived Domain for Efficient Site-Specific Photocoupling to Fc of Mouse IgG1

作者: Feifan Yu , Peter Järver , Per-Åke Nygren

DOI: 10.1371/JOURNAL.PONE.0056597

关键词: ChemistryImmunoglobulin Fc FragmentsProtein domainImmunoglobulin bindingBinding siteAntibodyImmunoglobulin GProtein engineeringBiochemistryBiotinylation

摘要: … engineering can be used to increase the interaction strength between the Z domain and Fc of mIgG 1 . … The resulting proteins were tested for binding to mIgG 1 and a preferred variant …

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