Sequential transcription of the genes of the lactose operon and its regulation by protein synthesis.

摘要: Abstract The initial kinetics of induction and the steady state rates synthesis β-galactosidase thiogalactoside transacetylase in Escherichia coli were examined under a variety conditions. Kinetic experiments with 5-fluorouracil carbon starvation suggest that z gene is transcribed into messenger ribonucleic acid (mRNA) before gene. In general, when protein was impaired, formation mRNA (defined as enzyme-forming capacity) delayed, although (similarly defined) occurred at its usual time. When completely blocked, could be but not. To explain these findings, tentative scheme proposed which transcription controlled by rate ribosomes travel along completed portion mRNA. Since do not appear to critical for gene, it suggested RNA polymerase maybe able advance ahead leading ribosome certain distance. also required shorter exposure inducer than did transcription. Chloramphenicol, specifically delayed transacetylase, inhibited production more β-galactosidase. At an antibiotic concentration 2 µg per ml, continued, while inhibited.