Temperature-Mediated Alterations of the Plant Apoplast as a Mechanism of Intracellular Freezing Stress Avoidance

作者: Jun Liu

DOI:

关键词: ApoplastCold acclimationPectinMatrix (biology)Permeability (electromagnetism)BiophysicsBotanyCalciumChemistryCell wallPolysaccharide

摘要: ii ACKNOWLEDGEMENTS iii TABLE OF CONTENTS iv LIST TABLES vi FIGURES vii 1.0 INTRODUCTION 1 2.0 LITERATURE REVIEW 5 2.1 Freezing Stress 2.1.1 Process 2.1.2 Avoidance and Tolerance 9 2.2 Apoplast 12 2.2.1 The Structure of the 2.2.2 Function 16 2.2.3 Responses to Cold Acclimation Role in Resistance 17 2.3 Calcium 21 2.3.1 is an Essential Element Plants 2.3.2 22 2.4 Allium fistulosum L. 23 3.0 MATERIALS AND METHODS 25 3.1 Plant Materials Growth 3.2 Treatments 3.3 Analysis 27 3.4 Study Permeability 3.5 Pectin Methyl Esterification 28 3.5.1 Sample Preparation Fixation 3.5.2 by Immunohistochemistry 29 3.5.3 Determination PME Activity 3.6 Cell Wall Compositional 30 3.6.1 wall isolation 3.6.2 Matrix Polysaccharide composition 31 4.0 RESULTS DISCUSSION 33 4.1 4.2 36 4.3 Studies Apoplastic 41 4.4 Degree Methyl-esterification 44 4.5 46 4.5.1 Quantity Ligule Tissue

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