ENZYMATIC DEGRADATION OF HEPARIN AS A TOOL FOR STRUCTURAL ANALYSIS
作者： Alfred Linker , Peter Hovingh
DOI: 10.1016/B978-0-12-484850-4.50007-7
关键词: Organic chemistry 、 Polysaccharide 、 Heparinase 、 Chromatography 、 Sulfation 、 Chemistry 、 Nitrous acid 、 Sulfate 、 Degradation (geology) 、 Uronic acid 、 Periodate
摘要: Heparin fractions obtained originally from a variety of biological sources were degraded by Flavobacterial heparinase, and the products analyzed for structural details. In addition periodate oxidation nitrous acid degradation used on polysaccharides as well their enzymatic breakdown to gain further information about distribution uronic units sulfate substitution. Based these data an arrangement lower sulfated regions in otherwise higher polymer has been proposed.
sci-hub.st 参考文章(29)
R. L. Taylor, J. E. Shively, H. E. Conrad, J. A. Cifonelli, Uronic acid composition of heparins and heparan sulfates Biochemistry. ,vol. 12, pp. 3633- 3637 ,(1973) , 10.1021/BI00743A010
John Hopwood, Magnus Höök, Alfred Linker, Ulf Lindahl, Anticoagulant activity of heparin: Isolation of antithrombin-binding sites FEBS Letters. ,vol. 69, pp. 51- 54 ,(1976) , 10.1016/0014-5793(76)80651-5
L.H. Lam, J.E. Silbert, R.D. Rosenberg, The separation of active and inactive forms of heparin Biochemical and Biophysical Research Communications. ,vol. 69, pp. 570- 577 ,(1976) , 10.1016/0006-291X(76)90558-1
Alfred Linker, Peter Hovingh, Isolation and characterization of oligosaccharides obtained from heparin by the action of heparinase. Biochemistry. ,vol. 11, pp. 563- 568 ,(1972) , 10.1021/BI00754A013
Sally S. Twining, Arthur S. Brecher, Characterization of the interaction between chymotrypsin and heparin Canadian Journal of Biochemistry. ,vol. 55, pp. 134- 139 ,(1977) , 10.1139/O77-021
Terence Painter, Bjørn Larsen, Jan Sjövall, L. Kääriäinen, S. E. Rasmussen, Erling Sunde, Nils Andreas Sørensen, Formation of Hemiacetals between Neighbouring Hexuronic Acid Residues during the Periodate Oxidation of Alginate. Acta Chemica Scandinavica. ,vol. 24, pp. 813- 833 ,(1970) , 10.3891/ACTA.CHEM.SCAND.24-0813
Magnus Höök, Ingemar Björk, John Hopwood, Ulf Lindahl, Anticoagulant activity of heparin: Separation of high-activity and low-activity heparin species by affinity chromatography on immobilized antithrombin FEBS Letters. ,vol. 66, pp. 90- 93 ,(1976) , 10.1016/0014-5793(76)80592-3
L.B. Jaques, The mast cells in the light of new knowledge of heparin and sulfated mucopolysaccharides General Pharmacology-the Vascular System. ,vol. 6, pp. 235- 245 ,(1975) , 10.1016/0306-3623(75)90017-8
John E. Shively, H. Edward Conrad, Formation of anhydrosugars in the chemical depolymerization of heparin. Biochemistry. ,vol. 15, pp. 3932- 3942 ,(1976) , 10.1021/BI00663A005
Zensaku Yosizawa, A new type of heparin “ω-heparin” isolated from whale organs Biochemical and Biophysical Research Communications. ,vol. 16, pp. 336- 341 ,(1964) , 10.1016/0006-291X(64)90036-1