A Bienzyme Electrode (Alkaline Phosphatase−Polyphenol Oxidase) for the Amperometric Determination of Phosphate

摘要: The electropolymerization of an alkaline phosphatase (AP)−amphiphilic pyrrole ammonium mixture previously adsorbed on the electrode surface provides efficient entrapment AP in a polypyrrole film. resulting biosensor was applied to detection phenyl phosphate via amperometric oxidation at 0.6 V vs SCE enzymically generated phenol. sensitivity and limit were 862 μA M-1 cm-2 20 μM, respectively. co-immobilization polyphenol oxidase leads bienzyme for determination basis o-quinone −0.2 V. An amplification factor 9.7 calculated as ratio monoenzyme sensitivities. In presence 5 mM MgCl2, 36.82 mA 0.2 Phosphate competitively inhibited hydrolysis phosphate. Consequently, its d...