Membrane mass spectrometer inlet for quantitation of nitric oxide

摘要: Nitric oxide (NO) is an important physiological and biochemical messenger that may be involved in endogenous carcinogenesis cell toxicity via formation of N-nitroso compounds or direct DNA damage by nitrosating agents arising from the reaction NO with O2. To study O2 model systems disappearance more such as cultures, we adapted optimized a membrane mass spectrometer inlet specifically for analyses. The consisted Silastic tubing inserted into Swagelok 'tee', which was attached to tuning probe. Kinetics can followed under electron impact conditions until NO2 interferes NO+ during fragmentation NO2+. aqueous concentration minimum detection determined 1.4 microM. response time step changes concentrations 7.0 s, fast enough permit real-time measurements upon addition Finally, depletion observed presence relative steady state responses inlets designed gas samples, their times, are explained analysis based on transfer theory.