Detection and fate of Bacillus anthracis (Sterne) vegetative cells and spores added to bulk tank milk.

摘要: A preparation of Bacillus anthracis (Sterne strain) spores was used to evaluate commercially available reagents and portable equipment for detecting anthrax contamination by using real-time PCR assess the fate added directly bulk tank milk. The Ruggedized Advanced Pathogen Identification Device (RAPID) employed detect in raw milk down a concentration 2,500 per ml. Commercially primers probes developed either protective antigen gene or lethal factor both provided easily read positive signals with RAPID following extraction from DNA kit. Nucleotide sequence analysis vrrA use extracted spiked molecular data that readily identified as B. 100% BLAST match Sterne Ames strains distinguished them cereus. Physical-fate thermal-stability studies demonstrated vegetative cells have strong affinity cream fraction whole single treatment at standard pasteurization temperatures, while cells, had no effect on spore viability even 14 days after treatment. Twenty-four hours first treatment, second 72 degrees C 15 s reduced population ca. 99% but still did not kill all spores. From these studies, we conclude techniques would little pasteurized poses obstacles rapid detection techniques.