Dual effect of DLBCL-derived EXOs in lymphoma to improve DC vaccine efficacy in vitro while favor tumorgenesis in vivo.

摘要: Exosomes derived from tumor cells (TEXs) are involved in both immune suppression, angiogenesis, metastasis and anticancer stimulatory, but the biological characteristics role of diffuse large B cell lymphoma (DLBCL)-derived exosomes have been less investigated. (EXOs) were isolated OCI-LY3, SU-DHL-16, Raji EXOs investigated using electron microscopy, flow cytometry analysis, Western blot analysis. The protein expression was determined by an antibody array. Next, communication between cell, stromal dendritic (DCs), T evaluated. Finally, effect DLBCL TEXs on growth vivo We demonstrated that lines displayed malignancy molecules such as c-Myc, Bcl-2, Mcl-1, CD19, CD20. There a different pattern Burkitt TEXs. easily captured DCs cells, mainly acted immunosuppressive mediator, evidenced induction apoptosis upregulation PD-1 cells. Furthermore, stimulated not only proliferation, migration also angiogenesis. As result, promoted vivo. On other hand, did induce DCs. After pulsed with TEXs, could stimulate clonal expansion increase secretion IL-6 TNFα, decrease production cytokine IL-4 IL-10. bearing mice immunized TEX shown to possess superior antilymphoma potency relative immunization lysates. This study provides framework for novel immunotherapies targeting DLBCL.