An investigation into the effects of X-ray on the recovery of ancient DNA from skeletal remains

摘要: Abstract The application of radiographic imaging methods like conventional X-Ray and computed tomography (CT) in bioarchaeological research is normally considered to be non-invasive. While this holds true on the macro- microscopic level, little known about potentially induced damage molecular level that could inhibit successful recovery ancient DNA (aDNA) from such specimens. Although there has been speculation concerning possible recovered remains following exposure radiation, published. Past studies attempted determine specific effect X-ray amplification bone recently butchered animals. results suggested clinical radiation decreased aDNA, un-dehydrated state samples might have biased results. In study we utilized dry human archaeological bones nine prehistoric historic individuals exposed them different levels using more accurately examine issue. Employing Next Generation Sequencing (NGS) shotgun sequencing libraries, quantitative PCR (qPCR), multiplex autosomal genetic markers show neither dosages (moderate irradiation) used nor 20-fold increased (strong a significant quantity quality can these We conclude bioarchaeology does not impair success subsequent aDNA if simple precautions are followed.