Poly(ethylene oxide) Grafted with Short Polyethylenimine Gives DNA Polyplexes with Superior Colloidal Stability, Low Cytotoxicity, and Potent In Vitro Gene Transfection under Serum Conditions
作者： Meng Zheng , Zhihong Zhong , Lei Zhou , Fenghua Meng , Rui Peng
DOI: 10.1021/BM2017965
关键词: Polymer chemistry 、 Gel electrophoresis 、 Dynamic light scattering 、 Molecular mass 、 Ethylene oxide 、 Copolymer 、 Zeta potential 、 Surface charge 、 Chemistry 、 Polyethylenimine
摘要: Poly(ethylene oxide) grafted with 1.8 kDa branched polyethylenimine (PEO-g-PEI) copolymers varying compositions, that is, PEO(13k)-g-10PEI, PEO(24k)-g-10PEI, and PEO(13k)-g-22PEI, were prepared investigated for in vitro nonviral gene transfer. Gel electrophoresis assays showed PEO(13k)-g-22PEI could completely inhibit DNA migration at an N/P ratio of 4/1, 3/1, respectively. Dynamic light scattering (DLS) zeta potential measurements revealed all three graft able to effectively condense into small-sized (80–245 nm) particles moderate positive surface charges (+7.2 ∼ +24.1 mV) ratios ranging from 5/1 40/1. The polyplex sizes zeta-potentials intimately depended on PEO molecular weights PEI densities. Notably, unlike 25 control, PEO-g-PEI polyplexes stable against aggregation under physiological salt as well 20% serum conditions due the shielding effect PEO. MTT ...
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