The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei

摘要: Species of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability compete for nutrients and inhibit or kill targets through the production antibiotics and/or hydrolytic enzymes. In addition these abilities, spp. have beneficial effects plants, including stimulation defenses promotion growth. Here we study role in T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), shikimate pathway branch point leading aromatic amino acids, which are not only essential components protein synthesis but also precursors wide range secondary metabolites. We isolated transformants with gene silenced. Compared wild-type, decreased levels expression silenced were accompanied by reduced CM activity, lower growth rates on different culture media, mycoparasitic behavior phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum Botrytis cinerea dual cultures. By contrast, higher amounts metabolites tyrosol, 2-phenylethanol salicylic acid detected supernatants from transformants, able F. B. cinerea. vitro assays, Tparo7-silenced showed capacity colonize tomato roots. The effect Tparo7-silencing responses was examined greenhouse assays. plants colonized exhibited an increased susceptibility comparison observed control plants. addition, turned yellowish defective jasmonic acid- ethylene-regulated signaling pathways seen analysis lipoxygenase 1 (LOX1), ethylene-insensitive 2 (EIN2) pathogenesis-related (PR-1) genes.