BLM unfolds G-quadruplexes in different structural environments through different mechanisms
作者： Wen-Qiang Wu , Xi-Miao Hou , Ming Li , Shuo-Xing Dou , Xu-Guang Xi
DOI: 10.1093/NAR/GKV361
关键词: Genetics 、 G-quadruplex 、 Förster resonance energy transfer 、 Bloom syndrome 、 DNA 、 Gene 、 Helicase 、 Mutation 、 Adenosine triphosphate 、 Biophysics 、 Biology
摘要: Mutations in the RecQ DNA helicase gene BLM give rise to Bloom's syndrome, which is a rare autosomal recessive disorder characterized by genetic instability and cancer predisposition. highly active binding unwinding G-quadruplexes (G4s), are physiological targets for BLM, as revealed genome-wide characterizations of expression cells from BS patients. With smFRET assays, we studied molecular mechanism BLM-catalyzed G4 unfolding showed that ATP required unfolding. Surprisingly, depending on environments G4, unfolds through different mechanisms: harboring 3′-ssDNA tail three discrete steps with unidirectional translocation, connected dsDNA ssDNA repetitive manner remains anchored at ss/dsDNA junction, was unfolded reeling ssDNA. This indicates one molecule may unfold G4s mechanisms.
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Mohamed M. Abdelhaleem, Helicases : methods and protocols Humana Press. ,(2010)
Ruobo Zhou, Jichuan Zhang, Matthew L Bochman, Virginia A Zakian, Taekjip Ha, Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA eLife. ,vol. 3, ,(2014) , 10.7554/ELIFE.02190
Wai Kit Chu, Ian D. Hickson, RecQ helicases: multifunctional genome caretakers Nature Reviews Cancer. ,vol. 9, pp. 644- 654 ,(2009) , 10.1038/NRC2682
Nathan A. Ellis, Joanna Groden, Tian-Zhang Ye, Joel Straughen, David J. Lennon, Susan Ciocci, Maria Proytcheva, James German, The Bloom's syndrome gene product is homologous to RecQ helicases Cell. ,vol. 83, pp. 655- 666 ,(1995) , 10.1016/0092-8674(95)90105-1
L. Ying, J. J. Green, H. Li, D. Klenerman, S. Balasubramanian, Studies on the structure and dynamics of the human telomeric G quadruplex by single-molecule fluorescence resonance energy transfer Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 14629- 14634 ,(2003) , 10.1073/PNAS.2433350100
Katrin Paeschke, John A. Capra, Virginia A. Zakian, DNA Replication through G-Quadruplex Motifs Is Promoted by the Saccharomyces cerevisiae Pif1 DNA Helicase Cell. ,vol. 145, pp. 678- 691 ,(2011) , 10.1016/J.CELL.2011.04.015
Aurore Guédin, Julien Gros, Patrizia Alberti, Jean-Louis Mergny, How long is too long? Effects of loop size on G-quadruplex stability Nucleic Acids Research. ,vol. 38, pp. 7858- 7868 ,(2010) , 10.1093/NAR/GKQ639
Michael K. Swan, Valerie Legris, Adam Tanner, Philip M. Reaper, Sarah Vial, Rebecca Bordas, John R. Pollard, Peter A. Charlton, Julian M. C. Golec, Jay A. Bertrand, Structure of human Bloom's syndrome helicase in complex with ADP and duplex DNA Acta Crystallographica Section D-biological Crystallography. ,vol. 70, pp. 1465- 1475 ,(2014) , 10.1107/S139900471400501X
R.-B. Guo, P. Rigolet, H. Ren, B. Zhang, X.-D. Zhang, S.-X. Dou, P.-Y. Wang, M. Amor-Gueret, X. G. Xi, Structural and functional analyses of disease-causing missense mutations in Bloom syndrome protein Nucleic Acids Research. ,vol. 35, pp. 6297- 6310 ,(2007) , 10.1093/NAR/GKM536
Sujoy Chatterjee, Jennifer Zagelbaum, Pavel Savitsky, Andreas Sturzenegger, Diana Huttner, Pavel Janscak, Ian D. Hickson, Opher Gileadi, Eli Rothenberg, Mechanistic insight into the interaction of BLM helicase with intra-strand G-quadruplex structures. Nature Communications. ,vol. 5, pp. 5556- 5556 ,(2014) , 10.1038/NCOMMS6556