SIMPLIFICATION OF THE ANTHRONE METHOD FOR THE DETERMINATION OF INULIN IN CLEARANCE STUDIES.

作者: Marvin A. Sackner , Warren D. Davidson

DOI: 10.5555/URI:PII:0022214363900738

关键词: Sodium hydroxideIncubationRenal functionChemistryInulinReagentChromatographyAnthroneDiphenylamine

摘要: Abstract The anthrone method of Young and Raisz 1 was modified by a decrease in the concentration reagent from 0.4 per cent to 0.08 reduction temperature for color development 75 °C. 38 Under these conditions, interference glucose is minimized so that inulin can be determined directly without prior removal incubation samples with yeast or sodium hydroxide. compared standard diphenylamine series clinical studies. mean ratio clearances (C In anthrone/C diphenylamine) 12 patients 0.996 (S.D. = ± 0.026). These data confirm earlier findings Walser, Davidson, Orloffs alkali-stable has same clearance as alkali-labile inulin.

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