Visualization of DNA and Protein–DNA Complexes with Atomic Force Microscopy
作者： Yuri L. Lyubchenko , Alexander A. Gall , Luda S. Shlyakhtenko
DOI: 10.1007/978-1-62703-776-1_17
关键词: Combinatorial chemistry 、 DNA 、 Nucleoprotein 、 Nucleic acid 、 Microscopy 、 Divalent 、 Ionic bonding 、 Chemistry 、 Mica 、 Sample preparation
摘要: This article describes sample preparation techniques for AFM imaging of DNA and protein-DNA complexes. The approach is based on chemical functionalization the mica surface with aminopropyl silatrane (APS) to yield an APS-mica surface. binds nucleic acids nucleoprotein complexes in a wide range ionic strengths, absence divalent cations, broad pH. chapter methodologies surfaces samples imaging. protocol synthesis purification APS also provided. applications are illustrated examples images
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