Carbonic anhydrase IX as a specific biomarker for clear cell renal cell carcinoma: comparative study of Western blot and immunohistochemistry and implications for diagnosis.
作者： José M. Giménez-Bachs , Antonio S. Salinas-Sánchez , Leticia Serrano-Oviedo , Syong H. Nam-Cha , Antonio Rubio-Del Campo
DOI: 10.3109/00365599.2012.685493
关键词: Carbonic Anhydrase IX 、 Western blot 、 Nephrectomy 、 Cancer 、 Medicine 、 Pathology 、 Biomarker (medicine) 、 Immunohistochemistry 、 Stage (cooking) 、 Clear cell renal cell carcinoma
摘要: Abstract Objective. This study aimed to evaluate the usefulness of carbonic anhydrase IX (CA-IX) expression in clear cell renal carcinoma (CCRCC) using two different techniques detect protein expression. Material and methods. An experimental, cross-sectional, analytical was conducted analyse proteins tumour healthy tissue specimens from 38 consecutive patients who underwent nephrectomy for cancer. CA-IX measured by immunohistochemistry Western blot analysis quantified. Statistical performed with positive negative specific agreements kappa coefficient. The sensitivity specificity both were assessed. tests association between quantitation normal prognosis factors (TNM stage Fuhrman nuclear grade), only CCRCC. Results. mean patient age 65 years, 78.9% men 57.9% tumours expre...
sci-hub.se 参考文章(31)
Murakami, Kanda, Tsuji, Kanayama, Kagawa, MN/CA9 gene expression as a potential biomarker in renal cell carcinoma BJUI. ,vol. 83, pp. 743- 747 ,(2001) , 10.1046/J.1464-410X.1999.00007.X
John N. Eble, Pathology and genetics of tumours of the urinary system and male genital organs IARC Press. ,(2004)
B??rje Ljungberg, Prognostic markers in renal cell carcinoma. Current Opinion in Urology. ,vol. 17, pp. 303- 308 ,(2007) , 10.1097/MOU.0B013E328277F180
René Opavský, Silvia Pastoreková, Vladimı́r Zelnı́k, Adriana Gibadulinová, Eric J. Stanbridge, Jan Závada, Richard Kettmann, Jaromı́r Pastorek, Human MN/CA9 gene, a novel member of the carbonic anhydrase family: Structure and exon to protein domain relationships Genomics. ,vol. 33, pp. 480- 487 ,(1996) , 10.1006/GENO.1996.0223
Johanna Sandlund, Egbert Oosterwijk, Kjell Grankvist, Jeannette Oosterwijk-Wakka, Börje Ljungberg, Torgny Rasmuson, Prognostic impact of carbonic anhydrase IX expression in human renal cell carcinoma BJU International. ,vol. 100, pp. 556- 560 ,(2007) , 10.1111/J.1464-410X.2007.07006.X
Andrew N. Young, Viraj A. Master, Gladell P. Paner, May D. Wang, Mahul B. Amin, Renal Epithelial Neoplasms: Diagnostic Applications of Gene Expression Profiling Advances in Anatomic Pathology. ,vol. 15, pp. 28- 38 ,(2008) , 10.1097/PAP.0B013E3181594720
S. V. Ivanov, I. Kuzmin, M.-H. Wei, S. Pack, L. Geil, B. E. Johnson, E. J. Stanbridge, M. I. Lerman, Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 12596- 12601 ,(1998) , 10.1073/PNAS.95.21.12596
John T. Leppert, John S. Lam, Allan J. Pantuck, Robert A. Figlin, Arie S. Belldegrun, Carbonic anhydrase IX and the future of molecular markers in renal cell carcinoma BJU International. ,vol. 96, pp. 281- 285 ,(2005) , 10.1111/J.1464-410X.2005.05615.X
Andrew N. Young, Jennifer Dale, Qiqin Yin-Goen, Wayne B. Harris, John A. Petros, Milton W. Datta, May D. Wang, Fray F. Marshall, Mahul B. Amin, Current trends in molecular classification of adult renal tumors Urology. ,vol. 67, pp. 873- 880 ,(2006) , 10.1016/J.UROLOGY.2005.11.042
Sergey Ivanov, Shu-Yuan Liao, Alla Ivanova, Alla Danilkovitch-Miagkova, Nadezhda Tarasova, Gregor Weirich, Marsha J. Merrill, Martin A. Proescholdt, Edward H. Oldfield, Joshua Lee, Jan Zavada, Abdul Waheed, William Sly, Michael I. Lerman, Eric J. Stanbridge, Expression of Hypoxia-Inducible Cell-Surface Transmembrane Carbonic Anhydrases in Human Cancer The American Journal of Pathology. ,vol. 158, pp. 905- 919 ,(2001) , 10.1016/S0002-9440(10)64038-2