Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells

摘要: Calcium carbonate, especially with nanostructure, has been considered as a good candidate material for bone regeneration due to its excellent biodegradability and osteoconductivity. In this study, rod-like calcium carbonate nanoparticles (Rod-CC NPs) desired water dispersibility were achieved the regulation of poly (acrylic acid). Characterization results revealed that Rod-CC NPs had an average length 240 nm, width 90 nm aspect ratio 2.60 negative ζ-potential −22.25 ± 0.35 mV. The degradation study illustrated degraded 23% at pH 7.4 45% 5.6 in phosphate-buffered saline (PBS) solution within three months. When cultured MC3T3-E1 cells, exhibited positive effect on proliferation osteoblast cells. Alkaline phosphatase (ALP) activity assays together osteocalcin (OCN) sialoprotein (BSP) expression observations demonstrated could induce differentiation Our developed well-dispersed which have great potential be used regeneration.