MicroRNA-365 promotes apoptosis in human melanoma cell A375 treated with hydatid cyst fluid of Echinococcus granulosus sensu stricto

摘要: Abstract Hydatid cyst fluid (HCF)-based therapeutics has experimentally targeted approaches for treating human cancer cell lines. MicroRNA-365 (miR-365) been reported to be an important tumor suppressor miRNA in cancers. However, it remains unknown, how miR-365 plays a pivotal role inducing apoptosis HCF-treated cells vitro. The fertile/infertile HCF was aspirated from liver of infected sheep and terms molecular taxonomy identified as G1 genotype Echinococcus granulosus sensu stricto. A375 melanoma were cultured into two groups: fertile infertile A375 cells. To assess the cytotoxicity various concentrations on cells, viability determined by using MTT assay. IC50 value A375 cells 85 μg/mL. Caspase-3 enzymatic activity evaluated fluorometric assay cells. In addition, mRNA expression Bax, Bcl-2, Caspase-9 qRT-PCR. Findings showed that 85 μg/mL 100 μg/mL have highest mortality (50%–52%) during 24 h. fold change Bax/Bcl-2 ratio, Caspase-9, higher compared treated normal epithelial (as control cell). conclusion, we over-expressed A375 cells, via treatment HCF. Our findings suggested high might negative regulator growth through activation pro-apoptotic are essential intrinsic apoptotic pathway. These provide new insights use HCF-derived metabolites design drug therapies vivo vaccine combat progression.