Hybrid superparamagnetic iron oxide nanoparticle-branched polyethylenimine magnetoplexes for gene transfection of vascular endothelial cells.
作者： Ran Namgung , Kaushik Singha , Mi Kyung Yu , Sangyong Jon , Yong Sook Kim
DOI: 10.1016/J.BIOMATERIALS.2010.01.123
关键词: Transfection 、 Gene delivery 、 Viability assay 、 Magnetofection 、 Biophysics 、 Biochemistry 、 Confocal microscopy 、 Transduction (genetics) 、 Polyethylene glycol 、 Materials science 、 Polyethylenimine
摘要: The work demonstrated the development of thermally cross-linked superparamagnetic nanomaterial which possessed polyethylene glycol moiety and covalently linked branched polyethylenimine (BPEI), exhibited highly efficient magnetofection even under serum conditioned media. study showed its high anti-biofouling, cell viability stability thus revealed a potential magnetic nanoparticle-mediated targeted gene delivery system. This particle mediated rapid transfection in primary vascular endothelial cells (HUVEC) successfully inhibits expression PAI-1 is responsible for various dysfunctions such as inflammation atherosclerosis thereby provides strategy to transfect sensitive HUVEC. sequential steps enhanced had been studied by monitoring cellular uptake with aid confocal microscopy.
postech.ac.kr
elsevier.com参考文章(30)
Giuseppina Caligiuri, Mats Rudling, Véronique Ollivier, Marie-Paule Jacob, Jean-Baptiste Michel, Göran K. Hansson, Antonino Nicoletti, Interleukin-10 deficiency increases atherosclerosis, thrombosis, and low-density lipoproteins in apolipoprotein E knockout mice. Molecular Medicine. ,vol. 9, pp. 10- 17 ,(2003) , 10.1007/BF03402102
Laura De Laporte, Jennifer Cruz Rea, Lonnie D. Shea, Design of modular non-viral gene therapy vectors Biomaterials. ,vol. 27, pp. 947- 954 ,(2006) , 10.1016/J.BIOMATERIALS.2005.09.036
Jennifer E. Phillips, Charles A. Gersbach, Andrés J. García, Virus-based gene therapy strategies for bone regeneration. Biomaterials. ,vol. 28, pp. 211- 229 ,(2007) , 10.1016/J.BIOMATERIALS.2006.07.032
H Herweijer, J A Wolff, Gene therapy progress and prospects : Hydrodynamic gene delivery Gene Therapy. ,vol. 14, pp. 99- 107 ,(2007) , 10.1038/SJ.GT.3302891
Yong Sook Kim, Youngkeun Ahn, Moon Hwa Hong, Soo Yeon Joo, Kye Hun Kim, Il Suk Sohn, Hyung Wook Park, Young Joon Hong, Ju Han Kim, Weon Kim, Myung Ho Jeong, Jeong Gwan Cho, Jong Chun Park, Jung Chaee Kang, Curcumin attenuates inflammatory responses of TNF-alpha-stimulated human endothelial cells. Journal of Cardiovascular Pharmacology. ,vol. 50, pp. 41- 49 ,(2007) , 10.1097/FJC.0B013E31805559B9
S F Aliño, A Crespo, F Dasí, Long-term therapeutic levels of human alpha-1 antitrypsin in plasma after hydrodynamic injection of nonviral DNA. Gene Therapy. ,vol. 10, pp. 1672- 1679 ,(2003) , 10.1038/SJ.GT.3302065
R. G. Webster, D. H. Fuller, J. R. Haynes, J. C. Santoro, H. L. Robinson, E. F. Fynan, DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 11478- 11482 ,(1993) , 10.1073/PNAS.90.24.11478
Christian Plank, Ulrike Schillinger, Franz Scherer, Christian Bergemann, J-S Rémy, Florian Krötz, Martina Anton, Jim Lausier, Joseph Rosenecker, None, The magnetofection method: using magnetic force to enhance gene delivery. Biological Chemistry. ,vol. 384, pp. 737- 747 ,(2003) , 10.1515/BC.2003.082
Daniel W. Pack, Allan S. Hoffman, Suzie Pun, Patrick S. Stayton, Design and development of polymers for gene delivery Nature Reviews Drug Discovery. ,vol. 4, pp. 581- 593 ,(2005) , 10.1038/NRD1775
Ran Namgung, Jihoon Kim, Kaushik Singha, Chun Ho Kim, Won Jong Kim, Synergistic Effect of Low Cytotoxic Linear Polyethylenimine and Multiarm Polyethylene Glycol: Study of Physicochemical Properties and In Vitro Gene Transfection Molecular Pharmaceutics. ,vol. 6, pp. 1826- 1835 ,(2009) , 10.1021/MP900096U